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Rurouni Kenshin: Ishin Shishi no Requiem

Walking around in Yokohama, Kenshin, Sanosuke, Kaoru and Yahiko see a confrontation between some drunken western sailors and a Japanese woman. An armed samurai steps in front to protect the woman and soon a small riot breaks out with the samurai winning. The woman is actually the sister of a person Kenshin had killed at the end of the last dynasty. The samurai who introduces himself as Shigure Takimi is unknown to Kenshin, he is trying to start a new revolution to overthrow the Meiji government.